A modified plate assay for screening phosphate solubilizing microorganisms.

Effects of Synthetic Pyrethroids on Phosphate Solubilizing Activity of Microorganisms

Pesticides are the important agrochemicals used for prevention of crops from pests. Their use has been largely increased in last few decades. The application of pesticides starts from the pre sowing stage. Different treatments include soil application, seed treatment, foliar spray, etc. Repeated applications of pesticides contaminate the soil. Soil is the most important site of biological inter...

Microplate Diffusion Assay for Screening of β-Glucanase-Producing Microorganisms

A method is described to screen fungal strains rapidly for overexpression of extracellular β-1,4-endoglucanase in the presence of high levels of sugar compounds. The semi-quantitative assay utilizes microplates in a 96-well format and an azurine dye covalently cross-linked (AZCL) chromogenic substrate. The digestion of AZCL-hydroxyethyl-β-1,4-endoglucanase results in the release of a blue dye d...

Effects of phosphate-solubilizing bacteria, native microorganisms, and rock dust on Jatropha curcas L. growth.

Microorganisms with the ability to release nutrients to the soil from insoluble sources may be useful for plant cultivation. We evaluated the growth-promoting effect on Jatropha curcas L. of phosphate-solubilizing bacteria (PSB) and the native microbiota in soil with or without rock dust. J. curcas L. is important for biodiesel production. The experiments were performed in a greenhouse under a ...

The biological assay of penicillin by a modified plate method.

Screening and identification of Iranian native phosphate solubilizing bacteria and investigation of their genetic diversity using RAPD markers

Phosphorus, the most essential nutrient for plants, becomes quickly unavailable for the plants in the soil. Phosphate solubilizing bacteria (PSB( can play an important role in providing Phosphorus for plants. In this study, the PSBs were screened from plant rhizosphere by Pikovskaya method. Then, the growth rate and phosphate solubilizing ability of 9 superior strains were measured at different...